Leaf anatomical characteristics and physiological responses to short-term drought in Ziziphusmauritiana (Lamk.)     

Manoj Kulkarni  Bert Schneider  Eran Raveh  Noemi Tel-Zur 《Scientia Horticulturae》2010

The aim of this work was to study the association between leaf anatomical characteristics and response to short-term drought stress in Ziziphusmauritiana Lamk. Six Z. mauritiana cultivars (Seb, Gola, Umran, Keitly, Q-29 and B-5/4) under field conditions in Israel's Negev desert were studied. Width of palisade mesophyll, spongy mesophyll and epidermis, xylem number and diameter in mid-vein were investigated with light microscopy. Short-term (3 weeks) drought stress tolerance was evaluated by monitoring plant response (leaf transpiration, diffusive resistance, chlorophyll fluorescence, leaf water potential and leaf relative water content). Greater epidermis and mesophyll widths and xylem diameters and densities were associated with increased tolerance to short-term water deficit expressed by preliminary wilting symptoms and proportional differences between initial and final physiological parameters. Significantly larger differences were found in Keitly, Umran and B-5/4 than in Seb, Gola and Q-29, indicating that the former cultivars are more sensitive to drought stress. Our results indicate that tolerance in descending order was Seb, Q-29, Gola, B-5/4, Keitly and finally Umran. The existence of an association between anatomical characteristics and short-term drought stress tolerance based on physiological responses is suggested.  相似文献   

Penetrative and dislodgeable residue characteristics of 14C-insecticides in apple fruit     

Mota-Sanchez D  Cregg B  Hoffmann E  Flore J  Wise JC 《Journal of agricultural and food chemistry》2012,60(12):2958-2966

Infinite- and finite-dose laboratory experiments were used to study the penetrative and dislodgeable residue characteristics of (14)C-insecticides in apple fruit. The differences in dislodgeable and penetrated residues of three radiolabeled insecticides ((14)C-thiamethoxam, (14)C-thiacloprid, and (14)C-indoxacarb), applied in aqueous solution with commercial formulations, were determined after water and methanol wash extractions. The rate of sorption and extent of penetration into the fruit cuticles and hypanthium of two apple cultivars were measured after 1, 6, and 24 h of treatment exposure, using radioactivity quantification methods. For all three compounds, 97% or more of the treatment solutions were found on the fruit surface as some form of non-sorbed residues. For indoxacarb, sorption into the epicuticle was rapid but desorption into the fruit hypanthium was delayed, indicative of a lipophilic penetration pathway. For the neonicotinoids, initial cuticular penetration was slower but with no such delay in desorption into the hypanthium.  相似文献   

Human exposure to perfluorinated compounds in Catalonia, Spain: contribution of drinking water and fish and shellfish     

Domingo JL  Ericson-Jogsten I  Perelló G  Nadal M  Van Bavel B  Kärrman A 《Journal of agricultural and food chemistry》2012,60(17):4408-4415

In this study, the concentrations of 15 perfluorinated compounds (PFCs) were analyzed in 30 water samples collected in Catalonia (Spain) at three stages of the drinking water treatment process in several water purification plants. In addition, the concentrations of 13 PFCs were determined in samples of fish and shellfish collected from coastal areas of Catalonia. The intake of PFCs through both pathways, drinking water intake and fish and shellfish consumption, was also estimated. In water samples, the highest mean concentrations corresponded to perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) (1.81 and 2.40 ng/L, respectively), whereas perfluorodecanosulfonate (PFDS) and perfluorotetradecanoic acid (PFTDA) were under their respective limits of detection in all analyzed samples. The results show that although the current treatment processes caused slight reductions in PFC concentrations, these processes did not mean significant changes in the amounts of PFCs already contained in the raw water. Among the analyzed PFCs in fish and shellfish, only seven compounds could be detected in at least one composite sample. PFOS showed the highest mean concentration (2.70 ng/g fw), being detected in all species with the exception of mussels. With regard to PFOA (mean, 0.074 ng/g fw), the highest concentrations were detected in prawn and hake (0.098 and 0.091 ng/g fw, respectively). The current exposure to PFCs through consumption of fish and shellfish indicates that it should not be of concern for the consumers. The amounts ingested are well below the recommended tolerable daily intakes, at least for those PFCs for which information is available.  相似文献   

Assignments of proton populations in dough and bread using NMR relaxometry of starch, gluten, and flour model systems     

Bosmans GM  Lagrain B  Deleu LJ  Fierens E  Hills BP  Delcour JA 《Journal of agricultural and food chemistry》2012,60(21):5461-5470

Starch-water, gluten-water, and flour-water model systems as well as straight-dough bread were investigated with (1)H NMR relaxometry using free induction decay and Carr-Purcell-Meiboom-Gill pulse sequences. Depending on the degree of interaction between polymers and water, different proton populations could be distinguished. The starch protons in the starch-water model gain mobility owing to amylopectin crystal melting, granule swelling, and amylose leaching, whereas water protons lose mobility due to increased interaction with starch polymers. Heating of the gluten-water sample induces no pronounced changes in proton distributions. Heating changes the proton distributions of the flour-water and starch-water models in a similar way, implying that the changes are primarily attributable to starch gelatinization. Proton distributions of the heated flour-water model system and those of fresh bread crumb are very similar. This allows identifying the different proton populations in bread on the basis of the results from the model systems.  相似文献   

Controlled but pragmatic investigations of interventions for behavioural disturbances in dogs and cats     

Porzsolt F  Fink H  Bert B 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(9-10):382-385

Veterinarians are often directly involved in clinical studies or requested for information to help interpret their results. Therefore, it is reasonable to examine the reservoir of study methods. This article transfers methodological considerations from clinical research into veterinary medicine. The study question determines the appropriate study method. Recently a ten-step procedure was suggested for selection of appropriate study designs in humans. Based on this approach, a pragmatic study design was adapted to the conditions prevailing in interventional studies in dogs and cats with disturbed behaviour. The different concepts for clinical studies are introduced. Whether or not the design and the evaluation of pragmatic studies in dogs and cats with disturbed behaviour has been maintained and the prerequisites have thereby been fulfilled so that the obtained results are suitable to be applied under everyday conditions can be tested in eight steps. Using the pragmatic design the superiority of complex interventions can be investigated. The results of pragmatic studies help to substantiate a value judgement, i. e., the recommendation or rejection of a specific therapeutic intervention for a defined disease entity in a specific therapeutic setting. The goal of pragmatic studies is to obtain results appropriate for use in everyday situations. In conclusion, the suggested procedure is useful for the selection of the appropriate study designs for specific questions. This procedure is also suitable to test whether the conclusions of published study results coincide with the chosen methods.  相似文献   

Identification of isopeptide bonds in heat-treated wheat gluten peptides     

Rombouts I  Lagrain B  Brunnbauer M  Koehler P  Brijs K  Delcour JA 《Journal of agricultural and food chemistry》2011,59(4):1236-1243

Results in this paper confirm heat-induced isopeptide bond formation in wheat gluten. Heating (24 h, 130 °C) of wheat gluten [moisture content 7.4%] decreased its extractability in sodium dodecyl sulfate containing buffer (pH 6.8), even after reduction of disulfide (SS) bonds. Thus, both SS bonds and non-SS bonds were responsible for the extractability loss. Cross-links of the lysinoalanine and lanthionine type were not present in the heated samples, but heat treatment reduced levels of available amino groups. Heating of purified and alkylated high molecular weight glutenin subunits (HMW-GS) under similar conditions also resulted in extractability loss, demonstrating that cross-linking did not solely depend on the availability of cysteine or cystine. These observations indicated that heat treatment had induced isopeptide bond formation, resulting in larger and unextractable molecules. Heating HMW-GS lysine- and glutamine-containing peptides induced the formation of isopeptide bonds, thereby supporting the above hypothesis. The level of isopeptide bond formation increased with heating time.  相似文献   

Physiological response of temperature shocks in turbot and sole     

Atle Foss  Hans van de Vis  Wout Abbink  Bert Lambooij  Bjørn Roth 《Journal Of Applied Aquaculture》2019,31(1):34-47

In the present study, selected temperature drops were examined to investigate the effects of live chilling on stress and welfare in turbot and sole. This study demonstrated that rapid temperature drops from 11°C–12°C and 18°C–18.7°C to 4°C–0°C in turbot resulted in higher blood sodium and potassium at 0°C (164 mmol l^?1, 4.4 mmol ^?1) compared to 4°C (153 mmol l^?1, 3.1 mmol ^?1), indicating osmoregulatory disturbance. A rapid temperature drop from 18°C to 0°C in Senegal sole also resulted in higher blood sodium and potassium at 0°C (164 mmol l^?1, 4.8 mmol ^?1) compared to the control group at 18°C (157 mmol l^?1, 3.2 mmol ^?1). Based on present findings, we conclude that immersion in ice water will have a negative effect on the animal osmoregulatory capacity, and we recommend that turbot and sole are stunned before slaughter.  相似文献   

Legal fundamentals and practical accomplishments of abattoir and meat research in mammalian game animals     

R M Hadlok  F Bert 《Tier?rztliche Praxis》1988,16(4):343-350

Meat regulations also involve game inspection. The previous publication mainly dealt with the regulations and their practice concerning game intended for slaughter. The following publication deals with game hunted for food (deer: red deer, sika deer, fallow deer, roe deer; horned ungulates: european mufflon, chamois; wild boar; european hare; european rabbit) and also game such as the badger and the raccoon.  相似文献   

Cortex is driven by weak but synchronously active thalamocortical synapses     

Bruno RM  Sakmann B 《Science (New York, N.Y.)》2006,312(5780):1622-1627

Sensory stimuli reach the brain via the thalamocortical projection, a group of axons thought to be among the most powerful in the neocortex. Surprisingly, these axons account for only approximately 15% of synapses onto cortical neurons. The thalamocortical pathway might thus achieve its effectiveness via high-efficacy thalamocortical synapses or via amplification within cortical layer 4. In rat somatosensory cortex, we measured in vivo the excitatory postsynaptic potential evoked by a single synaptic connection and found that thalamocortical synapses have low efficacy. Convergent inputs, however, are both numerous and synchronous, and intracortical amplification is not required. Our results suggest a mechanism of cortical activation by which thalamic input alone can drive cortex.  相似文献